Construction and characterization of region-specific microdissection libraries and single-copy microclones for short arm of human chromosome 2
- Eleanor Roosevelt Institute for Cancer Research, Denver, CO (United States)
The short arm of human chromosome 2, comprising approximately 93 million bp, has been divided into four regions to construct region-specific microdissection libraries to facilitate physical mapping and gene cloning. These four regions include 2p23-p25 (designated 2P1), 2p21-p23 (2P2), 2p14-p16 (2P3), and 2p11-p13 (2P4). Together with three previously constructed microdissection libraries of 2P1, 2P2 and 2P4, a fourth library for the region 2P3 has been constructed and characterized to complete all four region-specific libraries for the entire 2p. The 2P3 library is very large, potentially comprising 1,000,000 recombinant microclones with insert sizes ranging between 50 and 800 bp and a mean of 250 bp. Approximately 40% of the microclones contain unique sequences. Of the 77 single-copy microclones analyzed, 66 clones (86%) hybridized to both human and chromosome 2 DNAs, indicating that they were derived from human and are chromosome 2 specific. The hybridizing HindIII genomic fragments for the 66 microclones have also been determined.
- DOE Contract Number:
- FG03-94ER61819
- OSTI ID:
- 86508
- Journal Information:
- Somatic Cell and Molecular Genetics, Journal Name: Somatic Cell and Molecular Genetics Journal Issue: 4 Vol. 20; ISSN 0740-7750; ISSN SCMGDN
- Country of Publication:
- United States
- Language:
- English
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