Region-specific microdissection library and single-copy microclones for human chromosome 2p11-p13

Journal Article · Tue Mar 01 04:00:00 EST 1994 · Somatic Cell and Molecular Genetics; (United States)

DOI:https://doi.org/10.1007/BF02290682· OSTI ID:6940635

Yu, Jingwei; Qi, Jianxin; Kao, Fa-Ten ^[1]

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1. Univ. of Colorado Health Sciences Center, Denver, CO (United States)

The authors report the construction and characterization of a region-specific microdissection library for human chromosome 2p11-p13. This library (designated 2P4 library) is large, comprising 600,000 recombinant microclones. Thirty to 40% of the clones contain unique sequences. The insert sizes range from 100 to 800 bp, with a mean of 380 bp. A subset of the microclones was selected, based on their weak or no hybridization to total human DNA, for further analysis. Of 50 single-copy microclones analyzed, 35 clones (70%) were derived from human and are chromosome 2-specific. The insert sizes and the hybridizing genomic HindIII fragments of these clones were also determined. The 2P3 microdissection library and the single-copy microclones from the library are useful in preparing STS (sequence-tagged site) to isolate corresponding YAC (yeast artificial chromosome) or other clones with large inserts and for isolating region-specific cDNA clones as candidate genes for cloning disease-related genes assigned to this region.

• DOE Contract Number: FG03-94ER61819
• OSTI ID: 6940635
• Journal Information: Somatic Cell and Molecular Genetics; (United States), Journal Name: Somatic Cell and Molecular Genetics; (United States) Journal Issue: 2 Vol. 20:2; ISSN 0740-7750; ISSN SCMGDN
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOTECHNOLOGY
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENETIC ENGINEERING
GENETIC MAPPING
HUMAN CHROMOSOME 2
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NUCLEIC ACID HYBRIDIZATION
STRUCTURAL CHEMICAL ANALYSIS