Synteny between the Pro/sup +/ marker and human glutamate oxaloacetate transaminase

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Somatic Cell Genet.; (United States)

DOI:https://doi.org/10.1007/BF01538666· OSTI ID:7216572

Jones, C

Chinese hamster ovary cells with a specific auxotrophy for proline were fused with human cells from a variety of sources and the resulting hybrids analyzed for human genetic markers. Of 63 hybrid clones examined, 27 possessed both proline and cytoplasmic glutamate oxaloacetate transaminase markers; 36 had neither; and no clones were found possessing one and not the other. These results constitute evidence that the proline and glutamate oxaloacetate transaminase markers are syntenic. Evidence for absence of synteny between these and a variety of other human genes is presented. Biochemical tracer experiments established that the proline biosynthetic pathway through glutamate has been restored in the Pro/sup +/ hybrids.

• Research Organization: Roosevelt Inst. for Cancer Research, Denver
• OSTI ID: 7216572
• Journal Information: Somatic Cell Genet.; (United States), Journal Name: Somatic Cell Genet.; (United States) Vol. 1:4; ISSN SCEGD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
AMINOTRANSFERASES
ANIMAL CELLS
ANIMALS
AZOLES
BIOLOGY
BIOSYNTHESIS
CARBOXYLIC ACIDS
ENZYMES
GENES
GENETICS
HAMSTERS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROLINE
PYRROLES
PYRROLIDINES
RODENTS
SYNTHESIS
TRANSFERASES
VERTEBRATES