Genetics of somatic mammalian cells: genetic, immunologic, and biochemical analysis with Chinese hamster cell hybrids containing selected human chromosomes

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.73.1.193· OSTI ID:7302930

Kao, F T; Jones, C; Puck, T T

Through hybridization of specific Chinese hamster cell auxotrophs with human cells and selection in media lacking the nutritional supplements required by the former cells, a series of stable hybrid clones can be prepared. These hybrids have genomes consisting of a common part, the complete or almost complete set of Chinese hamster chromosomes, plus a variable part, one or a few human chromosomes. The identity of the human chromosomes can be varied by utilizing different Chinese hamster auxotrophs and the appropriate selective media. The human chromosomes present can be determined by a combination of cytogenetic analysis with chromosome banding and testing for specific human marker genes. Hybrids containing single human chromosomes 11 and 12 and the combination of both 11 and 12 are described. The system appears to lend itself to various studies such as identification of human cell surface antigens, determination of their chromosomal loci, measurement of their distribution among cells of normal human tissues, study of interrelations among syntenic and asyntenic genes, and mutational analysis of the human genome.

• Research Organization: Univ. of Colorado, Denver
• OSTI ID: 7302930
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 73:1; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGY
CHEMISTRY
CHROMOSOMES
DATA ANALYSIS
GENETICS
HAMSTERS
HYBRIDIZATION
IMMUNOLOGY
MAMMALS
MUTATIONS
NUTRITION
RODENTS
VERTEBRATES