Division delay after low x-ray doses and treatment with cyclohexionide

Journal Article · Fri Apr 01 04:00:00 EST 1977 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574737· OSTI ID:7212819

Schneiderman, M H; Braby, L A; Roesch, W C

Radiation-induced division delay of Chinese hamster ovary cells located in G/sub 2/, and in G/sub 2/ between the cycloheximide and x-ray transition points, was measured by the mitotic cell selection technique. The mitotic yield (number of mitotic cells after treatment expressed as a fraction of the control) decreased with increasing radiation dose (4.5 to 34 rad). However, either because some cells were not delayed or because delayed cells recovered rapidly, the mitotic yield did not fall to zero. When cycloheximide was combined with radiation to prevent repair of the radiation damage, only cells which were past the cycloheximide transition point and not delayed by the radiation were selected. The location of the transition points determined from the combined drug plus low-dose radiation (4.5 to 34 rad) experiments indicate a dose-dependent relationship, with more cells delayed as the dose was increased. In addition, the transition point for cells treated with cycloheximide plus 150 rad of x rays was closer to division than the 150 rad of x rays alone. These results are discussed in light of a recent model for radiation-induced division delay proposed by Dewey and Highfield.

• Research Organization: Battelle Pacific Northwest Labs., Richland, WA
• OSTI ID: 7212819
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 70:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CYCLOHEXIMIDE
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
FUNGICIDES
HAMSTERS
IONIZING RADIATIONS
IRRADIATION
LOW DOSE IRRADIATION
MAMMALS
MITOTIC DELAY
PESTICIDES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
VERTEBRATES
X RADIATION