Effect of bleomycin and irradiation on G/sub 2/ progression

Conference · Sat Sep 01 04:00:00 EDT 1979 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

OSTI ID:5340154

Kimler, B F

The interaction of bleomycin and x-irradiation on the induction of G/sub 2/ delay in Chinese hamster ovary cells was investigated utilizing the mitotic selection procedure for cell cycle analysis. Following the addition of BLM, the number of cells selected in mitosis remained at control level for a refractory period and then decreased. The location of the transition point, i.e., the age in G/sub 2/ at which cells become refractory to a progression blockade, was concentration-dependent, ranging from the S/G/sub 2/ boundary at low concentrations to the G/sub 2//M boundary at high concentrations. Depending upon the concentration of the drug used and the duration of exposure, the mitotic rate either decreased to zero or else leveled off at some intermediate value and then recovered to the control level. The duration of BLM-induced division delay was thus dependent upon the concentration used and the duration of exposure. When cells were treated with pulses of bleomycin (10-500 ..mu..g/ml) in addition to x-irradiation, the mitotic rate declined as with exposure to x-ray alone. However, the recovery from radiation-induced division delay and the subsequent reappearance of mitotic cells in the selection window was delayed until the cells had recovered from their BLM-induced division delay. This implies that, in contrast to the synergistic effects observed for cell lethality, BLM and radiation do not interact in the production of a progression blockade and the resultant division delay.

• Research Organization: Univ. of Kansas Medical Center, Kansas City
• OSTI ID: 5340154
• Conference Information: Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Journal Volume: 5:9
• Country of Publication: United States
• Language: English

Related Subjects

550603 -- Medicine-- External Radiation in Therapy-- (1980-)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLEOMYCIN
BODY
CELL CULTURES
CELL CYCLE
CELL DIVISION
DATA
DATA FORMS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FEMALE GENITALS
GONADS
HAMSTERS
INFORMATION
IONIZING RADIATIONS
ISOLATED VALUES
MAMMALS
MITOSIS
MITOTIC DELAY
NUMERICAL DATA
ORGANS
OVARIES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RODENTS
TIME DEPENDENCE
VERTEBRATES
X RADIATION