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Application of mammalian cytogenetics to mutagenicity studies. [Triethylone melamine, x radiation, gamma radiation, LET]

Conference ·
OSTI ID:7211824
Studies on induction of chromosome damage in germ cells by triethylene melamine (TEM) included determination of frequencies of chromosomal aberrations observed in human leukocytes after treating different stages of the cell cycle with TEM, frequencies of chromatid aberrations in metaphase I oocytes and the female pronuclear chromosomes following treatment of female mice with TEM, and frequencies of labeled diplotene-diakinesis figures and chromosome abberations at various intervals after treatment of primary spermatocytes with TEM and /sup 3/H-thymidine. Studies on effects of low linear energy transfer radiation on mouse oocytes showed that the frequency of aberrations increased as a function of time and remained constant 8 to 9 days post-exposure. It was concluded that cytogenetic procedures were adequate to evaluate certain mutagenic end points. (HLW)
Research Organization:
Oak Ridge National Lab., Tenn. (USA)
DOE Contract Number:
W-7405-ENG-26
OSTI ID:
7211824
Report Number(s):
CONF-770718-4
Country of Publication:
United States
Language:
English