Effects of radiations on DNA and repair of the damage. Progress report, May 1, 1976--March 31, 1977. [Escherichia coli, gamma radiation, UV radiation]
Last year's report that repair of DNA double-strand breaks from gamma rays occurs in E. coli was verified by additional experiments. Such repair requires recA function and the presence of another DNA molecule of the same base sequence, so it may involve a recombination-like event. Ultraviolet light acting on DNA containing bromouracil produces double-strand breaks by single photochemical events, and a single model can explain this as well as other results. Strains of E. coli which are unusually mutable by bromouracil--uvrE, mutL, mutR, mutS, are defective in mismatch repair. This strengthens the suggestion in last year's report that such mutagenesis occurs when enzymes responsible for the removal of mismatched bases are unable to remove all the mismatches. Ultraviolet mutagenesis of lambda phage may be a useful model for the study of mutagenesis in cells, because the effects of lesions in the gene mutated (i.e., in the phage) and changes in enzyme systems (by treating the host cells) can be examined separately. Quantitative data support this approach.
- Research Organization:
- Yale Univ., New Haven, Conn. (USA)
- DOE Contract Number:
- EY-76-S-02-3571
- OSTI ID:
- 7211823
- Report Number(s):
- COO-3571-17
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of radiations on DNA and repair of the damage. Progress report, May 1, 1974--June 30, 1977. [Gamma and UV radiation, Escherichia coli, lambda phage]
Double-strand breaks from single photochemical events in DNA containing 5-bromouracil. [Escherichia coli; uv radiation]
Repair of DNA double-strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light
Technical Report
·
Fri Dec 31 23:00:00 EST 1976
·
OSTI ID:7210876
Double-strand breaks from single photochemical events in DNA containing 5-bromouracil. [Escherichia coli; uv radiation]
Journal Article
·
Thu Nov 30 23:00:00 EST 1978
· Biophys. J.; (United States)
·
OSTI ID:6469972
Repair of DNA double-strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light
Journal Article
·
Mon Jun 01 00:00:00 EDT 1981
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6454622
Related Subjects
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GAMMA RADIATION
GENES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
MICROORGANISMS
MUTAGENESIS
NUCLEIC ACIDS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION
URACILS
VIRUSES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GAMMA RADIATION
GENES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
MICROORGANISMS
MUTAGENESIS
NUCLEIC ACIDS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION
URACILS
VIRUSES