Effects of. gamma. irradiation on embryonic bone and cartilage in vitro

Journal Article · Wed Feb 01 04:00:00 EST 1978 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574817· OSTI ID:7208142

Nijweide, P J ^[1]; Gaillard, P J; Hekkelman, J W; Herrmann-Erlee, M P.M.; van der Plas, A; Vegt, G B; Mellink, J H

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1. Leiden Univ., The Netherlands

In this study tissue culture methods were used to investigate the effects of ionizing irradiation on bone and cartilage of calvaria and radii of 18-day-old embryonic rats (resp. mice). The radii were studied histologically after 2 days of culture following the irradiation. A number of changes were observed. Decrease in growth of the bone collar and of the total rudiment together with an inhibition of the development of the marrow cavity could be established after a radiation absorbed dose of 500 to 1000 rad. The differences in length and sometimes in thickness of the bone collar between the control and the irradiated explants showed an inhibitory effect of irradiation on bone formation. This finding was corroborated by biochemical experiments with embryonic calvaria in which we found an inhibition of /sup 14/C-proline incorporation into the insoluble collagen fraction. In contrast with these findings we often found a stimulation of ectopic matrix deposition in the primitive marrow cavity of irradiated radii. An absorbed dose of 6000 rad did not appear to have a specific effect on the parathyroid hormone action at the receptor level but did damage a number of its physiological expressions.

• OSTI ID: 7208142
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 73:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE TISSUES
CARTILAGE
CONNECTIVE TISSUE
ELECTROMAGNETIC RADIATION
EMBRYOS
GAMMA RADIATION
IN VITRO
IONIZING RADIATIONS
IRRADIATION
MAMMALS
MICE
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
TISSUE CULTURES
TISSUES
VERTEBRATES