The Ah receptor nuclear translocator gene (ARNT) is located on q21 of human chromosome 1 and on mouse chromosome 3 near Cf-3

Journal Article · Wed Sep 01 00:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1377· OSTI ID:7199534

Johnson, B; Brooks, B A; Heinzmann, C ^[1]; Mohandas, T ^[2]

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1. Univ. of California, Los Angeles, CA (United States)
2. Harbor/UCLA Medical Center, Torrance, CA (United States)

The authors have mapped the Ah (aryl hydrocarbon) receptor nuclear translocator (ARNT) gene to a conserved linkage group located on mouse chromosome 3 and human chromosome 1. EcoRi-digested DNA from a panel of 17 human x mouse somatic cell hybrids was probed with a cDNA fragment of the human ARNT gene. Six of the 17 independent mouse x human hybrids were positive for human bands. Human chromosome 1 showed complete cosegregation with the gene, whereas discordant segregation was observed for all other human chromosomes. The human gene was localized to 1q21 by using DNA from mouse x human hybrid clones that retain translocations involving human chromosome 1, by segregation analysis in nine informative CEPH families, and by in situ hybridization. The mouse homologue was mapped to mouse chromosome 3 using a panel of 16 hamster x mouse somatic cell hybrids. Six of 16 mouse x hamster hybrids were positive for mouse bands, showing complete concordance with mouse chromosome 3. The mouse Arnt gene was regionally mapped on chromosome 3, using linkage analysis in an interspecific backcross. The results indicate that the mouse gene resides about 40 cM from the centromere and about 10 cM proximal to Cf-3, the gene for tissue factor. 41 refs., 4 figs., 5 tabs.

• OSTI ID: 7199534
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:3; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
CHROMOSOMES
ENZYMES
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 1
HUMAN CHROMOSOMES
HYDROCARBONS
HYDROLASES
HYDROXYLASES
MAPPING
MEMBRANE PROTEINS
METABOLISM
ORGANIC COMPOUNDS
OXIDOREDUCTASES
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
RECEPTORS
TRANSLOCATION