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Assignment of human thyrotropin-releasing hormone (TRH) receptor gene to chromosome 8

Journal Article · · Somatic Cell and Molecular Genetics; (United States)
DOI:https://doi.org/10.1007/BF01233384· OSTI ID:7285471

The human gene encoding thyrotropin-releasing hormone receptor was assigned to chromosome 8, using human-Chinese hamster ovary somatic cell hybrids, analyzed by Southern hybridizations. Hybridization was carried out with a [sup 32]P-labeled fragment of the human TRH-R genomic DNA. Hybridization of this probe to a human specific 10.5-kb DNA fragment of EcoRI-digested WBC DNA was used to localize the human TRH-R gene. No hybridization, by contrast, was seen with this probe and hamster DNA after EcoRI treatment. Results from 18 somatic cell hybrids corroborated unequivocally that the human TRH-R gene can be assigned to human chromosome 8.

OSTI ID:
7285471
Journal Information:
Somatic Cell and Molecular Genetics; (United States), Journal Name: Somatic Cell and Molecular Genetics; (United States) Vol. 19:6; ISSN 0740-7750; ISSN SCMGDN
Country of Publication:
United States
Language:
English