Differential regulation of oligodendrocyte markers by glucocorticoids: Post-transcriptional regulation of both proteolipid protein and myelin basic protein and transcriptional regulation of glycerol phosphate dehydrogenase

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.17.6807· OSTI ID:7195241

Kumar, S; Cole, R; Chiappelli, F; De Vellis, J ^[1]

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1. Univ. of California, Los Angeles (USA)

During neonatal development glucocorticoids potentiate oligodendrocyte differentiation and myelinogenesis by regulating the expression of myelin basic protein, proteolipid protein, and glycerol phosphate dehydrogenase. The actual locus at which hydrocortisone exerts its developmental influence on glial physiology is, however, not well understood. Gycerol phosphate dehydrogenase is glucocorticoid-inducible in oligodendrocytes at all stages of development both in vivo and in vitro. In newborn rat cerebral cultures, between 9 and 15 days in vitro, a 2- to 3-fold increase in myelin basic protein and proteolipid protein mRNA levels occurs in oligodendrocytes within 12 hr of hydrocortisone treatment. Immunostaining demonstrates that this increase in mRNAs is followed by a 2- to 3-fold increase in the protein levels within 24 hr. In vitro transcription assays performed with oligodendrocyte nuclei show an 11-fold increase in the transcriptional activity of glycerol phosphate dehydrogenase in response to hydrocortisone but no increase in transcription of myelin basic protein or proteolipid protein. These results indicate that during early myelinogeneis, glucocorticoids influence the expression of key oligodendroglial markers by different processes: The expression of glycerol phosphate dehydrogenase is regulated at the transcriptional level, whereas the expression of myelin basic protein and proteolipid protein is modulated via a different, yet uncharacterized, mechanism involving post-transcriptional regulation.

• OSTI ID: 7195241
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:17; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MARKERS
BODY
BRAIN
CELL CONSTITUENTS
CELL DIFFERENTIATION
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CEREBRUM
CHEMISTRY
CORTICOSTEROIDS
CYTOCHEMISTRY
ENZYMES
GENE REGULATION
GLUCOCORTICOIDS
HEMIACETAL DEHYDROGENASES
HYDROXY COMPOUNDS
KETONES
MAMMALS
MEMBRANES
MESSENGER-RNA
MYELIN
NERVE CELLS
NERVOUS SYSTEM
NUCLEIC ACIDS
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PREGNANES
PROTEINS
RATS
RNA
RODENTS
SOMATIC CELLS
STEROIDS
VERTEBRATES