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In vivo acylation of proteolipid protein and DM-20 in myelin and myelin subfractions of developing rat brain: immunoblot identification of acylated PLP and DM-20

Journal Article · · Neurochem. Res.; (United States)
DOI:https://doi.org/10.1007/BF00964704· OSTI ID:5394955
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The acylation of proteolipid protein (PLP) was examined in myelin and myelin subfractions from rat brain during the active period of myelination. Proteolipid protein and DM-20 in myelin and myelin subfractions were readily acylated in developing rat brain 22 hours after intracerebral injection of (/sup 3/H)palmitic acid. No differences in the relative specific activity of PLP in myelin from 9-, 15-, and 30-day-old rat brains was observed; however, the relative specific activity of PLP in the heavy myelin subfraction tended to be higher than that in the light myelin subfraction. The acylation of PLP was confirmed by fluorography of immuno-stained cellulose nitrate sheets, clearly establishing that the acylated protein is in fact the oligodendroglial cell- and myelin-specific protein, PLP. Since PLP is acylated in the 9-day-old animal, when little compact myelin is present, it is possible that the acylation of PLP is a prerequisite for the incorporation of this protein into the myelin membrane.
Research Organization:
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri
OSTI ID:
5394955
Journal Information:
Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 8:5; ISSN NERED
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550801 -- Morphology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOSYNTHESIS
BODY
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LIPIDS
LIPOPROTEINS
MAMMALS
MYELIN
NERVOUS SYSTEM
ONTOGENESIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RATS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES