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Characterization of cultured rat oligodendrocytes proliferating in a serum-free, chemically defined medium

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
;
A serumless, chemically defined medium has been developed for the culture of oligodendrocytes isolated from primary neonatal rat cerebral cultures. Combined together, insulin, transferrin, and fibroblast growth factor synergistically induced an essentially homogeneous population (95-98%) of cells expressing glycerol-3-phosphate dehydrogenase activity to undergo cell division. Proliferating cells were characterized by several criteria: (i) ultrastructural analysis by transmission electron microscopy identified the cell type as an oligodendrocyte; (ii) biochemical assays showed expression of three oligodendrocyte biochemical markers, induction of both glycerol phosphate dehydrogenase and lactate dehydrogenase, and presence of 2',3'-cyclic nucleotide 3'-phosphodiesterase; and (iii) immunocytochemical staining showed cultures to be 95-98% positive for glycerol phosphate dehydrogenase, 90% for myelin basic protein, 60-70% for galactocerebroside, and 70% for A2B5.
Research Organization:
Univ. of California, Los Angeles
DOE Contract Number:
AC03-76SF00012
OSTI ID:
6085359
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 82:10; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMISTRY
BIOLOGY
BODY
BRAIN
CELL PROLIFERATION
CENTRAL NERVOUS SYSTEM
CEREBRUM
CHEMISTRY
CULTURE MEDIA
CYTOLOGY
ELECTRON MICROSCOPY
ENZYMES
ESTERASES
HEMIACETAL DEHYDROGENASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
LABELLED COMPOUNDS
LACTATE DEHYDROGENASE
MAMMALS
MICROSCOPY
MORPHOLOGICAL CHANGES
NEONATES
NERVE CELLS
NERVOUS SYSTEM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHOSPHODIESTERASES
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SOMATIC CELLS
THYMIDINE
TRANSMISSION ELECTRON MICROSCOPY
TRITIUM COMPOUNDS
ULTRASTRUCTURAL CHANGES
VERTEBRATES