Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata

Journal Article · Sat May 01 04:00:00 EDT 1976 · J. Bacteriol.; (United States)

OSTI ID:7195109

Yen, H C; Marrs, B

The recently discovered gene transfer system of Rhodopseudomonas capsulata was used to construct a genetic map of a region concerned with bacteriochlorophyll and carotenoid production. Mutants blocked in the biosynthesis of these compounds were isolated, and each was characterized on the basis of pigments accumulated during growth under low pO/sub 2/. One-point, two-point, three-point, and ratio test crosses were performed between various mutant strains, and the results were amenable to conventional genetic analyses. A mapping function was found that related cotransfer frequency to map distance. Seven clusters of mutations, five affecting carotenoid and two affecting bacteriochlorophyll biosynthesis, were arranged in one linkage group. Each cluster of mutations is thought to represent a gene. The length of the mapped region is estimated to be less than 1 percent of the genome. Cotransfer is observed between markers separated by about 5 to 10 genes. (auth)

• Research Organization: St. Louis Univ.
• OSTI ID: 7195109
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 126:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGY
BIOSYNTHESIS
CARBOXYLIC ACIDS
CAROTENOIDS
CHLOROPHYLL
GENE RECOMBINATION
GENES
GENETICS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
MICROORGANISMS
MUTANTS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
SYNTHESIS
TERPENES