Variation of levels of mRNA coding for antenna and reaction center polypeptides in Rhodopseudomonas capsulata in response to changes in oxygen concentration

Journal Article · Thu Mar 01 04:00:00 EST 1984 · J. Bacteriol.; (United States)

OSTI ID:6168538

Clark, W G; Davidson, E; Marrs, B L

The effect of oxygen tension on the transcription of genes coding for the photosynthetic apparatus of Rhodopseudomonas capsulata was determined by the Southern hybridization technique. Restriction endonuclease digests of the R-prime plasmid pRPS404 and a subcloned fragment thereof served as DNA probes for genetically defined regions. The results showed that transcripts corresponding to the genes for certain pigment-binding polypeptides increase in amount by about 40-fold after a drop in oxygen tension. Transcripts hybridizing to genes involved in bacteriochlorophyll biosynthesis increase to a much lesser extent, and several genes involved in carotenoid biosynthesis are not affected by pO/sub 2/.

• Research Organization: Saint Louis Univ. School of Medicine, MO
• OSTI ID: 6168538
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 157:3; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHLOROPHYLL
DNA
ELEMENTS
GENES
GENETIC ENGINEERING
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
MEMBRANES
MESSENGER-RNA
NONMETALS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN
PEPTIDES
PHOTOSYNTHETIC BACTERIA
PHOTOSYNTHETIC MEMBRANES
PHYTOCHROMES
PIGMENTS
PLASMIDS
POLYPEPTIDES
PORPHYRINS
PROTEINS
RECOMBINANT DNA
RNA
SYNTHESIS
TRANSCRIPTION