Genetic-physical mapping of a photosynthetic gene cluster from R. capsulata

Journal Article · Sun Jul 01 04:00:00 EDT 1984 · Cell; (United States)

DOI:https://doi.org/10.1016/0092-8674(84)90428-8· OSTI ID:6654999

Zsebo, K M; Hearst, J E

Transposon mutagenesis and complementation analysis of the photosynthesis genes in Rhodopseudomonas capsulata is presented utilizing Tn5.7 mutagenized R-primes. The R-prime pRPS404 contains many of the genes necessary for the differentiation of the photosynthetic apparatus. Utilizing homologous recombination, 30 independent copies of Tn5.7 were inserted into the R. capsulata chromosome with subsequent deletion of wild-type alleles. Mutants were characterized by absorption spectroscopy, SDS-PAGE, and determination of capability for photosynthetic growth. Many mutations in the bacteriochlorophyll and carotenoid biosynthetic pathways were isolated. A regulator mutation was isolated affecting reaction-center synthesis as well as a 44 kd heme-containing polypeptide. Complementation analysis using various pRPR404::Tn5.7 plasmids has led to the postulation of transcriptional units. 32 references, 7 figures, 2 tables.

• Research Organization: Lawrence Berkeley Lab., CA
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6654999
• Journal Information: Cell; (United States), Journal Name: Cell; (United States) Vol. 37; ISSN CELLB
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTROSCOPY
BACTERIA
CELL CONSTITUENTS
CHEMICAL REACTIONS
DATA
ELECTROPHORESIS
EXPERIMENTAL DATA
GENE MUTATIONS
GENE RECOMBINATION
GENES
GENETIC MAPPING
INFORMATION
MAPPING
MICROORGANISMS
MUTATIONS
NUMERICAL DATA
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHOTOSYNTHETIC REACTION CENTERS
PLASMIDS
RHODOPSEUDOMONAS
SPECTROSCOPY
SYNTHESIS
TRANSCRIPTION