A unique enhancer element for the trans activator (p40 sup tax ) of human T-cell leukemia virus type I that is distinct from cyclic AMP- and 12-O-tetradecanoylphobol-13-acetate-responsive elements

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Journal of Virology; (USA)

OSTI ID:7195040

Fujisawa, Junichi; Toita, Masami; Yoshida, Mitsuaki ^[1]

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1. Cancer Institute, Tokyo (Japan)

The trans activator (p40{sup tax}) of human T-cell leukemia virus type I (HTLV-I) is a transcriptional factor that activates the long terminal repeat (LTR) of HTLV-I and interleukin-2 receptor {alpha}. The authors examined the HTLV-I enhancer responsible for tax-mediated trans activation and identified (A/T)(G/C)(G/C)CNNTGACG(T/A) as a plausible tax-responsive element (TRE). The putative TRE in the LTR was found to be different from the elements required for activation by cyclic AMP and 12-O-tetradecanoylphorbol-13-acetate, although these elements overlapped each other. The TRE was also different from a binding site of N-{kappa}B-like factor that was identified was identified in the interleukin-2 receptor {alpha} promoter and human immunodeficiency virus LTR as a TRE. The latter result was further demonstrated by the failure of the NF-{kappa}B sequence to compete with the TRE of the LTR in a protein-binding assay. These findings indicate that tax function and its cascade can modulate activities of various enhancer sequences, which are probably regulated by distinct DNA-binding factors.

• OSTI ID: 7195040
• Journal Information: Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 63:8; ISSN JOVIA; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CONSTITUENTS
CHEMICAL ACTIVATION
CHLORAMPHENICOL
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERS
GROWTH FACTORS
ISOTOPES
LEUKEMIA VIRUSES
LIGHT NUCLEI
LYMPHOKINES
MEMBRANE PROTEINS
MICROORGANISMS
MITOGENS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PROTEINS
RADIOISOTOPES
RECEPTORS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VIRUSES