Activation of endogenous c-fos proto-oncogene expression by human T-cell leukemia virus type I-encoded p40 sup tax protein in the human T-cell line, Jurkat

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Journal of Virology; (USA)

OSTI ID:7195254

Nagata, Kinya; Ohtani, Kiyoshi; Nakamura, Masataka; Sugamura, Kazuo ^[1]

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1. Tohoku Univ. School of Medicine, Sendai (Japan)

The authors examined the ability of the trans-acting factor p40{sup tax} of human T-cell leukemia virus type I (HTLV-I), which is thought to be a crucial molecule in T-cell transformation by HTLV-I, to activate expression of a set of endogenous cellular genes related to T-cell proliferation. For this purpose, they established a subclone (JPX-9) of Jurkat cells that was stably transfected with an expression plasmid containing the p40{sup tax} gene, whose expression is definitively dependent on heavy-metal ions. Expression of the interleukin-2 receptor {alpha} chain in JPX-9 cells was induced in response to the induction of p40{sup tax} expression, as has been demonstrated by others in transient transfection experiments with Jurkat cells. In addition, they found that significant enhancement of expression of the nuclear proto-oncogene c-fos was closely associated with expression of p40{sup tax}. Continuous enhancement in the level of c-fos mRNA was observed in the presence of p40{sup tax}. These results suggest that (i) in addition to the interleukin-2-interleukin-2 receptor system, cellular genes such as c-fos, which regulate normal T-cell growth, are also activated directly or indirectly by p40{sup tax} and (ii) p40{sup tax}-induced modulation of gene expression plays a crucial role in T-cell transformation by HTLV-I.

• OSTI ID: 7195254
• Journal Information: Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 63:8; ISSN 0022-538X; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL TRANSFORMATIONS
CHEMICAL ACTIVATION
CHEMISTRY
CONNECTIVE TISSUE CELLS
GENES
GROWTH FACTORS
LEUKEMIA VIRUSES
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MESSENGER-RNA
MICROORGANISMS
MITOGENS
NUCLEIC ACIDS
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PLASMIDS
PRIMATES
PROTEINS
RECEPTORS
RNA
SOMATIC CELLS
TUMOR CELLS
VERTEBRATES
VIRUSES