The adenovirus E4 gene, in addition to the E1A gene, is important for trans-activation of E2 transcription and for E2F activation

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Journal of Virology; (USA)

OSTI ID:7194985

Reichel, R; Neill, S D; Kovesdi, I; Simon, M C; Raychaudhuri, P; Nevins, J R ^[1]

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1. Duke Univ. Medical Center, Durham, NC (USA)

Previous experiments have demonstrated that adenovirus infection of human and mouse cells leads to an E1A-dependent activation of the DNA-binding capacity of a cellular transcription factor termed E2F. E2F binds to two sites in the adenovirus E2 early promoter which have been shown to be critical for E1A-dependent E2 early transcription, and the E2F-binding sites can confer E1A-induced transcription to a heterologous promoter. In addition, under a variety of circumstances, the increase in E2F-binding activity coincides with the activation of E2 transcription. The authors now find that, in addition to the E1A gene, another early viral gene, the E4 gene, is necessary for the activation of E2F-binding activity. Extracts prepared from human 293 cells, which express the E1A and E1B genes, had low levels of E2F activity, whereas infection of 293 cells with the E1A mutant dl312 increased E2F activity. A coinfection with the two mutants yielded the normal wild-type increase in E2F. Furthermore, infection of HeLa cells with a high multiplicity of dl312 did not yield an increase in E2F activity. Thus, it appears that both the E1A gene and the E4 gene are directly involved in E2F activation. Measurements of E2 RNA production in a dl366 infection as compared with a wild-type or dl312 infection demonstrate that the E4 gene is essential for full E2 transcription. They conclude that the activation of the E2F factor leading to the activation of E2 transcription requires the combined action of both the E1A 289-amino-acid protein and an E4 product.

• OSTI ID: 7194985
• Journal Information: Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 63:9; ISSN 0022-538X; ISSN JOVIA
• Country of Publication: United States
• Language: English

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550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
CHEMISTRY
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DNA
ENZYMES
GENE REGULATION
GENE REPRESSORS
GENES
HELA CELLS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MEMBRANE PROTEINS
MESSENGER-RNA
MICE
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POLYMERIZATION
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
RNA
RNA POLYMERASES
RODENTS
TRANSCRIPTION
TRANSFERASES
VERTEBRATES
VIRUSES