E1A-dependent trans-activation of the human MYC promoter is mediated by the E2F factor
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
- Duke Univ. Medical Center, Durham, NC (USA)
E2F is a cellular transcription factor that binds to two sites in the adenovirus E2 promoter. Previous experiments have implicated E2F in the E1A-dependent transactivation of the E2 gene since levels of active E2F increase markedly during adenovirus infection in parallel with the increase in E2 transcription, and an E2F binding site can confer E1A inducibility to a heterologous promoter. Here the authors show that E2F binds to two sequence elements within the P2 promoter of the human MYC gene which are within a region that is critical for promoter activity. The MYC promoter can be trans-activated in an E1A-dependent manner and site-directed mutagenesis demonstrates that these E2F elements are essential for trans-activation. Finally, they also find that adenovirus infection of quiescent cells results in a stimulation of the endogenous MYC gene. They conclude that the activation of the E2F factor, which is likely responsible for the activation of viral E2 transcription, is also responsible for the E1A-dependent induction of MYC transcription.
- OSTI ID:
- 5434023
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:10; ISSN 0027-8424; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
GENE REGULATION
GENE REPRESSORS
GENES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
METHYLATION
MICROORGANISMS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYMERIZATION
PRIMATES
PROTEINS
RADIOISOTOPES
TRANSCRIPTION
VERTEBRATES
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
GENE REGULATION
GENE REPRESSORS
GENES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
METHYLATION
MICROORGANISMS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYMERIZATION
PRIMATES
PROTEINS
RADIOISOTOPES
TRANSCRIPTION
VERTEBRATES
VIRUSES