Phosphoinositides metabolism in primary culture of dog thyroid cells: Effects of thyrotropin and carbachol

Journal Article · Sat Mar 31 23:00:00 EST 1990 · Metabolism, Clinical and Experimental; (USA)

DOI:https://doi.org/10.1016/0026-0495(90)90258-E· OSTI ID:7193224

Taguchi, M; Field, J B ^[1]

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1. St Luke's Episcopal Hospital, Houston, TX (USA)

Thyrotropin (TSH) and carbachol stimulated in a dose-dependent manner the accumulation of 3H-glycerophosphoinositol (GPI), 3H-inositol monophosphate (IP1), 3H-inositol bisphosphate (IP2) and 3H-inositol trisphosphate (IP3) in primary cultures of dog thyroid cells prelabeled with myo-(2-3H)inositol. TSH, 250 mU/mL, stimulated 3H-IP3 level after a 10-minute incubation while 10 mU/mL TSH increased it during a 60-minute incubation. The effect of carbachol was more rapid and greater than that of TSH. Carbachol, 100 mumol/L, elevated 3H-IP3 after a 2-minute incubation and 3H-IP3 formation was increased by as little as 1 mumol/L carbachol. TSH stimulation was observed only if the cells were deprived of TSH for 5 days before being labeled with 3H-inositol. Prolongation of the labeling period or addition of TSH, (Bu)2cAMP or carbachol during the labeling increased 3H-inositol incorporation into polyphoinositides (PIPs). When the cells were labeled without any other addition, control and TSH-stimulated 3H-IP3 levels increased in parallel with 3H-PIP levels. However, TSH or carbachol-stimulated 3H-IP3 levels did not increase in proportion to 3H-PIPs level when the cells were labeled with TSH or (Bu)2cAMP. Thus, the ratio of 3H-IP3/3H-PIPs (both control and TSH or carbachol-stimulated) decreased in the cells labeled with TSH or (Bu)2cAMP, which might reflect TSH stimulation of 3H-inositol incorporation into PIPs pool(s) that do not participate in hormone-induced hydrolysis of PIPs.

• OSTI ID: 7193224
• Journal Information: Metabolism, Clinical and Experimental; (USA), Journal Name: Metabolism, Clinical and Experimental; (USA) Vol. 39:4; ISSN METAA; ISSN 0026-0495
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CELL CULTURES
DOGS
DRUGS
ENDOCRINE GLANDS
ESTERS
GLANDS
HORMONES
HYDROGEN COMPOUNDS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KINETICS
LIPIDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PEPTIDE HORMONES
PHOSPHOLIPIDS
PITUITARY HORMONES
REACTION KINETICS
SACCHARIDES
THYROID
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TSH
VERTEBRATES