Soman-induced seizures impair norepinephrine-stimulated phosphoinositide turnover

Technical Report · Thu May 13 04:00:00 EDT 1993

OSTI ID:7300516

Filbert, M G; Phann, S; Forster, J; Ballough, G P; Cann, F J

Seizure activity increases turnover of phosphoinositide bisphosphate (PIP2). Turnover of PIP2 is thought to be modulated by neurotransmitter interactions. The effect of soman-induced seizures on neurotransmitter-stimulated PIP 2 turnover was examined in rats. Thirty minutes after induction of seizure activity, rats were euthanized and slices prepared from the hippocampus or cerebral cortex were incubated with myo-(2-3H) inositol for incorporation into phospholipids. Hydrolysis of phosphoinositides was determined by measuring the accumulation of (3H) inositol-l-phosphate (IP1) in the presence of LiCl. Carbachol, norepinephrine (NE) and high K+ increased accumulation of IP1 in slices from control rats. GABA was without effect on IP1 accumulation but potentiated the stimulation of PIP, hydrolysis by NE. NE-stimulated IP1 accumulation in slices from rats undergoing seizures was significantly reduced. GABA potentiation of the NE-stimulated hydrolysis was also reduced.

• Research Organization: Army Medical Research Inst. of Chemical Defense, Aberdeen Proving Ground, MD (United States)
• OSTI ID: 7300516
• Report Number(s): AD-P-008803/9/XAB
• Country of Publication: United States
• Language: English

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