Hybridizability of gamma-irradiated lactic dehydrogenase
Journal Article
·
· Radiat. Res.; (United States)
The hybridizabilities of the gamma-irradiated chicken heart and pig muscle lactic dehydrogenases were estimated by hybridizing the irradiated enzymes with the unirradiated pig heart lactic dehydrogenase. The disc gel electrophoretic patterns of the inter- and intraspecific hybrids showed that the LDH activity of the pig heart isozyme band increased as a function of dose. This observation was analyzed upon the binomial redistribution pattern of the recombined subunits. The result shows that the hybridizabilities of both the chicken heart and pig muscle isozymes decreased along with the loss of catalytic activity and the release from substrate inhibition. The titration of free SH groups of the irradiated chicken isozyme suggested that the unfolding of the peptide chain destroyed the specific tertiary structure needed for the binding of subunits. (auth)
- Research Organization:
- Kyoto Univ., Osaka
- OSTI ID:
- 7193047
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 65:3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Depression of Substrate Inhibition in Gamma Irradiated Lactic Dehydrogenase
Molecular cloning and functional expression in heterologous mammalian cells of the cDNAs encoding lactate dehydrogenase isozymes A (muscle), B (heart), and C (testis) from man and mouse
ON THE INFLUENCE OF WHOLE-BODY X IRRADIATION ON THE ACTIVITY OF LACTIC ACID DEHYDROGENASE IN BLOOD SERUM AND ORGAN EXTRACTS OF RATS. PART 2
Journal Article
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Wed Oct 31 23:00:00 EST 1973
· Radiation Research
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OSTI ID:4389947
Molecular cloning and functional expression in heterologous mammalian cells of the cDNAs encoding lactate dehydrogenase isozymes A (muscle), B (heart), and C (testis) from man and mouse
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6360835
ON THE INFLUENCE OF WHOLE-BODY X IRRADIATION ON THE ACTIVITY OF LACTIC ACID DEHYDROGENASE IN BLOOD SERUM AND ORGAN EXTRACTS OF RATS. PART 2
Journal Article
·
Sat Jun 01 00:00:00 EDT 1963
· Strahlentherapie (West Germany)
·
OSTI ID:4694858
Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIRDS
BODY
CARDIOVASCULAR SYSTEM
CHICKENS
DEHYDROGENASES
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ENZYMES
FOWL
GAMMA RADIATION
HEART
HYBRIDIZATION
IONIZING RADIATIONS
IRRADIATION
LACTATE DEHYDROGENASE
MAMMALS
MUSCLES
ORGANS
OXIDOREDUCTASES
RADIATION EFFECTS
RADIATIONS
SWINE
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIRDS
BODY
CARDIOVASCULAR SYSTEM
CHICKENS
DEHYDROGENASES
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ENZYMES
FOWL
GAMMA RADIATION
HEART
HYBRIDIZATION
IONIZING RADIATIONS
IRRADIATION
LACTATE DEHYDROGENASE
MAMMALS
MUSCLES
ORGANS
OXIDOREDUCTASES
RADIATION EFFECTS
RADIATIONS
SWINE
VERTEBRATES