Molecular cloning and functional expression in heterologous mammalian cells of the cDNAs encoding lactate dehydrogenase isozymes A (muscle), B (heart), and C (testis) from man and mouse

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6360835

Sakai, I; Hou, E W; Sharief, F S; Hiraoka, B Y; Takano, T; Li, S S.L.

The full-length cDNAs encoding human LHD-A (muscle), human and mouse LDH-B (heart), and mouse LDH-C (testis) isozymes have been isolated and their nucleotide sequences determined. The cDNAs for human LDH-A and mouse LDH-C isozymes were inserted into mammalian expression vectors and were shown to direct the synthesis of functional LDH isozymes in Chinese hamster ovary cells. The exogeneous LDH-A and LDH-C subunits were found to form hybrid LDH tetrameric isozymes with endogeneous LDH-A subunit of CHO cells. The genomic DNA containing LDH cDNA insert and the levels of mRNA expression are being characterized.

• Research Organization: NIEHS, Research Triangle Park, NC
• OSTI ID: 6360835
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
BODY
CARDIOVASCULAR SYSTEM
CHO CELLS
CLONING
DNA-CLONING
ENZYMES
GONADS
HEART
ISOENZYMES
MALE GENITALS
MAMMALS
MAN
MESSENGER-RNA
MICE
MOLECULAR STRUCTURE
MUSCLES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PRIMATES
RNA
RODENTS
TESTES
VERTEBRATES