Isolation and characterization of two cDNA clones of anaerobically induced lactate dehydrogenase from barley roots

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5628907

Hondred, D; Hanson, A D ^[1]

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1. Michigan State Univ., East Lansing (USA)

In barley roots during hypoxia, five lactate dehydrogenase (LDH) isozymes accumulate with a concomitant increase in enzyme activity ({approximately}20-fold). These isozymes are thought to be tetramers resulting from the random association of the products of two Ldh loci. To investigate this system, cDNA clones of LDH have been isolated from a {lambda}gt11 library using antiserum raised against barley LDH purified {approximately}3,000-fold and using nucleic acid probes synthesized by the polymerase chain reaction. Two cDNA clones were obtained (1,305 and 1,166 bp). The deduced amino acid sequences of the two barley LDHs are 96% identical to each other, and 50% and 40% identical to vertebrate and bacterial LDHs, respectively. Northern blots identified a single mRNA band ({approximately}1.5 kb) whose level rose 8-fold during hypoxia.

• OSTI ID: 5628907
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540110
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANOXIA
BARLEY
BIOLOGICAL EFFECTS
CEREALS
DNA POLYMERASES
ENZYMES
GRAMINEAE
LILIOPSIDA
MAGNOLIOPHYTA
MESSENGER-RNA
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
RNA
ROOTS
TRANSFERASES