Identification of the human pim-1 gene product as a 33-kilodalton cytoplasmic protein with tyrosine kinase activity
The human pim-1 gene was recently identified as a new putative oncogene located on chromosome 6p21, a region showing karyotypic abnormalities in particular leukemias. In the present work the authors characterized the pim protein product. In vitro translation of positively selected poly(A)/sup +/ mRNA indicates that this gene encodes a 33-kilodalton protein. Anti-pim antibodies were raised against a fused TrpE-pim protein induced in a bacterial expression vector. This antibody immunoprecipitated a 33-kilodalton protein from in vivo (/sup 35/S)methionine-labeled K562 and KCl myelogenous origin cell lines. This protein was localized to the cytoplasm, and in vivo labeling as well as in vitro kinase assay suggests that it is a phosphoprotein with tyrosine kinase activity. This was further confirmed by performing autophosphorylation directly on a p33/sup pim/-containing gel band cut out after sodium dodecyl sulfate-polyacrylamide gel electrphoresis. The results imply that the tyrosine kinase activity of pim can be recovered after boiling the pim-1 protein in sample buffer: a feature not described yet for this class of protein. These results suggest that pim-1 is a new member of the subgroup of oncogenes encoding tyrosine kinases.
- Research Organization:
- Institute of Interdisciplinary Research, Batiment C, Faculty of Medicine, Campus Erasme, Free Univ. of Brussels, Route de Lennik 808, Brussels 1070 (BE)
- OSTI ID:
- 7192083
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:4; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BIOSYNTHESIS
CARBOXYLIC ACIDS
DISEASES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
GENE RECOMBINATION PROTEINS
GENES
GENETIC MAPPING
HEMIC DISEASES
HYDROXY ACIDS
IMMUNOLOGY
IN VITRO
LEUKEMIA
MALES
MAMMALS
MAN
MAPPING
MEN
MESSENGER-RNA
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHOPROTEINS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PRIMATES
PROTEINS
RNA
SYNTHESIS
TRANSFERASES
TYROSINE
VERTEBRATES