Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases

Journal Article · Tue May 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.9.3328· OSTI ID:5323142

Kanner, S B; Reynolds, A B; Vines, R R; Parsons, J T ^[1]

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1. Univ. of Virginia Health Sciences Center, Charlottesville (USA)

Cellular transformation by oncogenic retroviruses encoding protein tyrosine kinases coincides with the tyrosine-specific phosphorylation of multiple protein substrates. Previous studies have shown that tyrosine phosphorylation of a protein of 120 kDa, p120, correlated with src transformation in chicken embryo fibroblasts. Additionally, the authors previously identified two phosphotyrosine-containing cellular proteins, p130 and p110, that formed stable complexes with activated variants of pp60{sup src}, the src-encoded tyrosine kinase. To study transformation-relevant tyrosine kinase substrates, they have generated monoclonal antibodies to individual tyrosine phosphoproteins, including p130, p120, p110, and five additional phosphoproteins (p210, p125, p118, p85, and p185/p64). These antibodies detected several of the same tyrosine phosphoproteins in chicken embryo fibroblasts transformed by avian retroviruses Y73 and CT10, encoding the yes and crk oncogenes, respectively. Protein substrates in mouse, rat, hamster, and human cells overexpressing activated variants of chicken pp60{sup src} were also detected by several of the monoclonal antibodies.

• OSTI ID: 5323142
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:9; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MONOCLONAL ANTIBODIES
RADIOASSAY
PHOSPHOTRANSFERASES
ENZYME IMMUNOASSAY
TYROSINE
PHOSPHORYLATION
CHICKENS
EMBRYONIC CELLS
IODINE 125
ONCOGENES
ONCOGENIC TRANSFORMATIONS
SUBSTRATES
VIRUSES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOASSAY
BIRDS
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FOWL
GENES
HYDROXY ACIDS
IMMUNOASSAY
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques