Rous sarcoma virus transforming protein tyrosine kinase is expressed and active in sarcoma-free avian embryos microinjected with Rous sarcoma virus

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.20.7587· OSTI ID:5672969

Howlett, A R; Carter, V C; Martin, G S; Bissell, M J ^[1]

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1. Lawrence Berkeley Lab., CA (USA)

Early embryonic avian tissue is resistant to transformation by Rous sarcoma virus. To determine the nature of this resistance, the authors examined the expression and properties of the Rous sarcoma virus transforming protein pp60{sup v-src}, in infected embryonic chicken limbs in ovo. Lysates from Rous sarcoma virus-infected limbs contained the viral structural protein p19{sup gag}, as detected by immunoblot analysis and showed pp60{sup v-src} kinase activity in vitro. Immunoblot analysis of lysates with anti-phosphotyrosine antibodies revealed a number of phosphotyrosine-containing proteins present in lysates of Rous sarcoma virus-infected embryos but not in lysates of control, uninfected embryos. These studies demonstrate that pp60{sup v-src} is co-expressed with viral structural determinants in infected embryonic avian tissue. The localization pattern of the major src gene substrate p36 (calpactin I) was compared with that of p19{sup gag} by double-label immunofluorescence and found to be generally nonoverlapping. These observations are consistent with the concept that the induction of tumors in ovo requires complementation between viral determinants and host factors. These host factors, which may be critical substrates of pp60{sup src}, are subject to developmental regulation in the avian embryo.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5672969
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:20; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOCHEMISTRY
BIRDS
CARBOXYLIC ACIDS
CHEMISTRY
CHICKENS
CYTOCHEMISTRY
EMBRYONIC CELLS
EMBRYOS
ENZYME ACTIVITY
ENZYMES
FOWL
HYDROXY ACIDS
IMMUNOLOGY
INJECTION
INTAKE
MICROORGANISMS
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PRECIPITATION
SEPARATION PROCESSES
TRANSFERASES
TYROSINE
VERTEBRATES
VIRUSES