Modulation of arachidonic acid metabolism by Rous sarcoma virus

Journal Article · Sat Jul 01 04:00:00 EDT 1989 · Journal of Virology; (USA)

OSTI ID:6968874

Barker, K; Aderem, A; Hanafusa, H ^[1]

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1. Rockefeller Univ., New York, NY (USA)

Arachidonic acid (C{sub 20:4}) metabolites were released constitutively from wild-type Rous sarcoma virus-transformed chicken embryo fibroblasts (CEF). {sup 3}H-labeled C{sub 20:4} and its metabolites were released from unstimulated and uninfected CEF only in response to stimuli such as serum, phorbol ester, or the calcium ionophore A23187. High-pressure liquid chromatography analysis showed that the radioactivity released from ({sup 3}H)arachidonate-labeled transformed cells was contained in free arachidonate and in the cyclooxygenase products prostaglandin E{sub 2} and prostaglandin F{sub 2} alpha; no lipoxygenase products were identified. The release of C{sub 20:4} and its metabolites from CEF infected with pp60{sup src} deletion mutants was correlated with serum-independent DNA synthesis and with the expression of the mRNA for 9E3, a gene expressed in Rous sarcoma virus-transformed cells which has homology with several mitogenic and inflammatory peptides. {sup 3}H-labeled C{sub 20:4} release was not correlated with p36 phosphorylation, which argues against a role for this protein as a phospholipase A{sub 2} inhibitor. CEF infected with other oncogenic viruses encoding a tyrosine kinase also released C{sub 20:4}, as did CEF infected with viruses that contained mos and ras; however, infection with a crk-containing virus did not result in stimulation of {sup 3}H-labeled C{sub 20:4} release, suggesting that utilization of this signaling pathway is specific for particular transformation stimuli.

• OSTI ID: 6968874
• Journal Information: Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 63:7; ISSN JOVIA; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ARACHIDONIC ACID
AZINES
BIOCHEMISTRY
BIRDS
CARBOXYLIC ACIDS
CARCINOGENS
CELL TRANSFORMATIONS
CHEMICAL REACTIONS
CHEMISTRY
CHICKENS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
EMBRYONIC CELLS
ESTERS
FIBROBLASTS
FOWL
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
METABOLITES
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MUTANTS
NUCLEOSIDES
NUCLEOTIDES
ONCOGENIC TRANSFORMATIONS
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PARASITES
PHORBOL ESTERS
PHOSPHOLIPIDS
PHOSPHORYLATION
PROSTAGLANDINS
PYRIMIDINES
RIBOSIDES
SEPARATION PROCESSES
SOMATIC CELLS
THIN-LAYER CHROMATOGRAPHY
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
VIRUSES