Band 3 tyrosine kinase in avian erythrocyte plasma membrane is immunologically related to pp60 sup c-src

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · American Journal of Physiology; (USA)

OSTI ID:5060177

Hillsgrove, D; Shores, C G; Parker, J C; Maness, P F ^[1]

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1. Univ. of North Carolina, Chapel Hill (USA)

The authors have identified in the plasma membrane of the chicken erythrocyte a 60-kDa tyrosine-specific protein kinase immunologically related to the transforming protein pp60{sup v-src} of Rous sarcoma virus. The erythrocyte protein kinase phosphorylated heavy chains of tumor-bearing rabbit (TBR) antibodies reactive with pp60{sup c-src} at tyrosine in immune complex protein kinase assays. The kinase was identified as a 60-kDa protein by ({sup 35}S)methionine labeling of erythrocytes and by autophosphorylation in immune complexes. The kinase migrated on two-dimensional gel electrophoresis with an apparent pI and molecular mass similar to pp60{sup c-src}. A plasma membrane-enriched fraction isolated from chicken red cells contained the majority of the kinase activity. Incubation of the plasma membrane fraction with ({sup 32}P)ATP resulted in tyrosine phosphorylation of the anion transport protein band 3. Band 3 phosphorylation was blocked by TBR antibodies, indicting that the kinase recognized by pp60{sup c-src} antibodies was responsible for band 3 phosphorylation. These results demonstrate that the avian erythrocyte plasma membrane contains a tightly bound tyrosine-specific protein kinase identical or closely related to pp60{sup c-src} and that this kinase is responsible for band 3 phosphorylation in vitro.

• OSTI ID: 5060177
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:2; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIONS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENZYMES
ERYTHROCYTES
EVEN-ODD NUCLEI
HYDROXY ACIDS
IONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
METHIONINE
MOLECULAR STRUCTURE
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
TRANSFERASES
TYROSINE
UPTAKE