Band 3 tyrosine kinase in avian erythrocyte plasma membrane is immunologically related to pp60 sup c-src

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · American Journal of Physiology; (USA)

OSTI ID:5060177

Hillsgrove, D; Shores, C G; Parker, J C; Maness, P F ^[1]

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1. Univ. of North Carolina, Chapel Hill (USA)

The authors have identified in the plasma membrane of the chicken erythrocyte a 60-kDa tyrosine-specific protein kinase immunologically related to the transforming protein pp60{sup v-src} of Rous sarcoma virus. The erythrocyte protein kinase phosphorylated heavy chains of tumor-bearing rabbit (TBR) antibodies reactive with pp60{sup c-src} at tyrosine in immune complex protein kinase assays. The kinase was identified as a 60-kDa protein by ({sup 35}S)methionine labeling of erythrocytes and by autophosphorylation in immune complexes. The kinase migrated on two-dimensional gel electrophoresis with an apparent pI and molecular mass similar to pp60{sup c-src}. A plasma membrane-enriched fraction isolated from chicken red cells contained the majority of the kinase activity. Incubation of the plasma membrane fraction with ({sup 32}P)ATP resulted in tyrosine phosphorylation of the anion transport protein band 3. Band 3 phosphorylation was blocked by TBR antibodies, indicting that the kinase recognized by pp60{sup c-src} antibodies was responsible for band 3 phosphorylation. These results demonstrate that the avian erythrocyte plasma membrane contains a tightly bound tyrosine-specific protein kinase identical or closely related to pp60{sup c-src} and that this kinase is responsible for band 3 phosphorylation in vitro.

• OSTI ID: 5060177
• Journal Information: American Journal of Physiology; (USA), Vol. 253:2; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIONS
UPTAKE
MEMBRANE PROTEINS
PHOSPHORYLATION
PHOSPHOTRANSFERASES
MOLECULAR STRUCTURE
ATP
CELL MEMBRANES
ELECTROPHORESIS
ERYTHROCYTES
METHIONINE
PHOSPHORUS 32
SULFUR 35
TYROSINE
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHARGED PARTICLES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
HYDROXY ACIDS
IONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
MEMBRANES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RADIOISOTOPES
SULFUR ISOTOPES
TRANSFERASES
550201* - Biochemistry- Tracer Techniques