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Characterization of the TPR-MET oncogene p65 and the MET protooncogene p140 protein-tyrosine kinases

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
DOI:https://doi.org/10.1073/pnas.85.1.21· OSTI ID:6464215
; ; ; ; ;

The proteins encoded by the human TPR-MET oncogene (p65/sup tpr-met/) and the human MET protooncogene (p140/sup met/) have been identified. The p65/sup tpr-met/ and p140/sup met/, as well as a truncated TPR-MET product expression in Escherichia coli, p50/sup met/, are autophosphorylated in vitro on tyrosine residues. Using the immunocomplex kinase assay, p140/sup met/ activity was detected in various human tumor epithelial cell lines. In vivo, p65/sup tpr-met/ is phosphorylated on both serine and tyrosine residues, while p140/sup met/ is phosphorylated on serine and threonine. p140/sup met/ is labeled by cell-surface iodination procedures, suggesting that it is a receptor-like transmembrane protein-tyrosine kinase.

Research Organization:
National Cancer Institute, Frederick, MD (USA)
OSTI ID:
6464215
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:1; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
EPITHELIUM
ESCHERICHIA COLI
EVEN-ODD NUCLEI
GENES
HALIDES
HALOGEN COMPOUNDS
HALOGENATION
HYDROXY ACIDS
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINATION
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
MEMBRANE PROTEINS
METHIONINE
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEI
ODD-EVEN NUCLEI
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
SERINE
SODIUM COMPOUNDS
SODIUM IODIDES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TISSUES
TRANSFERASES
TUMOR CELLS
TYROSINE
VERTEBRATES