Identification and biochemical characterization of p70/sup TRK/, product of the human TRK oncogene

Journal Article · Thu Oct 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.19.6707· OSTI ID:7242254

Mitra, G; Martin-Zanca, D; Barbacid, M

TRK is a human transforming gene generated in a colon carcinoma by a somatic rearrangement that fused a nonmuscle tropomyosin gene to sequences that shared extensive homology with members of the tyrosine-protein kinase supergene family. These sequences are likely to be derived from a transmembrane receptor gene whose putative ligand binding domain has been replaced by tropomyosin. In the present studies, the authors have expressed the entire coding sequences of the TRK oncogene as well as its protein kinase-related carboxyl-terminal domain in Escherichia coli. Antisera raised against these bacteria-synthesized TRK polypeptides has allowed us to identify the gene product of the TRK oncogene as a 70-kDa protein. Immunoprecipitates containing p70/sup TRK/ have an associated protein kinase activity specific for tyrosine residues. Moreover, p70/sup TRK/ is phosphorylated in vivo in serine (75%), threonine (20%), and tyrosine (5%) residues. Finally, immunofluorescence and cellular fractionation studies indicate that p70/sup TRK/ is preferentially located in the cytoplasmic fraction.

• Research Organization: Frederick Cancer Research Facility, MD (USA)
• OSTI ID: 7242254
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:19; ISSN PNASA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CARCINOMAS
CELL CONSTITUENTS
CYTOPLASM
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
DRUGS
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FLUORESCENCE
GENE RECOMBINATION
GENES
HYDROXY ACIDS
IN VIVO
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUMINESCENCE
MAMMALS
MAN
METHIONINE
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PRIMATES
PROTEINS
RADIOISOTOPES
SERINE
SUBCELLULAR DISTRIBUTION
SULFUR 35
SULFUR ISOTOPES
THREONINE
TRANSFERASES
TYROSINE
VERTEBRATES