Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro: II. Results with 20 chemicals

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850130108· OSTI ID:7190622

Loveday, K S; Lugo, M H ^[1]; Resnick, M A; Anderson, B E; Zeiger, E ^[2]

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1. American Biogenecis Corp., Woburn, MA (USA)
2. Institute of Environmental Health Sciences, Research Triangle Park, NC (USA)

Twenty chemicals were tested for their ability to induce sister chromatid exchanges (SCEs) and chromosomal aberrations (ABs) in cultured Chinese hamster ovary cells (CHO). These chemicals were tested with and without an added metabolic activation system (rat liver S9 fraction). Four chemicals were negative in both assays, 1 induced ABs only, and 15 were positive for SCEs; 6 of these 15 also induced ABs. The effect of cell harvest time on the ability to detect the induction of chromosomal aberrations was examined for six chemicals. Five of these had caused at least one of the following cell cycle delay, aberrations observed in first division metaphase cells in SCE assay, or a weak response in the standard AB assay (10-12-hr growth period). Three chemicals, chlorinated trisodium phosphate, 1,2-dibromo-3-chloropropane, and tetrakis(hydroxymethyl)phosphonium chloride, were positive using both the standard and extended harvest times. N-Nitrosodimethylamine and diphenhydramine HCl were only positive using an extended harvest time, and malonaldehyde was negative using both standard and extended harvest times.

• OSTI ID: 7190622
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 13:1; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AROMATICS
BENZENE
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CYCLE
CHLORINATED ALIPHATIC HYDROCARBONS
CHLORINATED AROMATIC HYDROCARBONS
CHO CELLS
CHROMOSOMAL ABERRATIONS
DRUGS
GENETIC EFFECTS
HALOGENATED ALIPHATIC HYDROCARBONS
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
IN VITRO
METABOLIC ACTIVATION
MITOMYCIN
MUTAGENS
MUTATION FREQUENCY
MUTATIONS
NITROSO COMPOUNDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SISTER CHROMATID EXCHANGES
SOLUBILITY