Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro III: Results with 27 chemicals

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850130208· OSTI ID:7005912

Gulati, D K ^[1]; Witt, K; Anderson, B; Zeiger, E; Shelby, M D ^[2]

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1. Environmental Health Research and Testing, Inc., Lexington, KY (USA)
2. National Institute of Environmental Health Sciences, Research Triangle Park, NC (USA)

Twenty-seven chemicals previously tested in rodent carcinogenicity assays were tested for induction of chromosomal aberrations (ABS) and sister chromatid exchanges (SCE) in Chinese hamster ovary (CHO) cells as part of a larger analysis of the correlation between results of in vitro genetic toxicity assays and carcinogenicity bioassays. Chemicals were tested up to toxic doses with and without exogenous metabolic activation. Seventeen of the chemicals tested were carcinogens; only two of these were negative for both ABS and SCE. Of the eight noncarcinogens tested, four were negative for both endpoints and four gave a positive response for at least one endpoint. Of the remaining two chemicals, one, diallylphthalate, gave an equivocal response in the bioassay and a positive response in these CHO cell cytogenetics tests. The other chemical, 2,4-toluene diisocyanate, was tested for carcinogenicity as a mixture with the 2,6-isomer; the mixture was carinogenic, but the cytogenetic test results for the 2,4-isomer were negative. Experiments with unsynchronized CHO cells demonstrated that mean SCE frequency increased with increasing culture time, and this may have been a factor in the positive results obtained for five chemicals in the SCE test under conditions of delayed harvest.

• OSTI ID: 7005912
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 13:2; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANILINE
ANIMAL CELLS
ANIMALS
AROMATICS
ASCORBIC ACID
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGY
BIPHENYL
BODY
CARBOXYLIC ACID SALTS
CARCINOGEN SCREENING
CARCINOGENESIS
CARCINOGENS
CELL CYCLE
CHO CELLS
CHROMOSOMAL ABERRATIONS
CYTOLOGY
DIGESTIVE SYSTEM
GENETIC EFFECTS
GLANDS
HYDROCARBONS
IN VITRO
LIVER
MAMMALS
METABOLIC ACTIVATION
MUTATIONS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHTHALATES
RATS
RODENTS
SCREENING
SISTER CHROMATID EXCHANGES
VERTEBRATES
VITAMINS