Conditions affecting the mutagenicity of trichloroethylene in Salmonella

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850130302· OSTI ID:7190592

McGregor, D B; Reynolds, D M ^[1]; Zeiger, E ^[2]

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1. Inveresk Research International, Musselburg (England)
2. National Institute of Environmental Health Sciences, Research Triangle Park, NC (USA)

Trichloroethylene (TCE) is a high production volume chemical frequency stabilized with oxiranes. These oxiranes may be responsible for the mutagenic activity of TCE in Salmonella, which has been occasionally, but not consistently, reported. High purity and oxirane-stabilized TCE samples were tested for their mutagenic potential in Salmonella typhimurium strains TA 1535, TA 98, and TA 100. Stabilized TCE was tested using a preincubation protocol up to a dose level of 10,000 {mu}g per plate, but not mutagenic response was observed in either the presence or absence of a supplementary metabolic activation system (S9 mix) derived from Aroclor 1254-induced male rat liver. TCE without oxirane stabilizers also was nonmutagenic when tested in a vapor delivery system at nominal concentrations of up to 20% and using S9 mix derived from either rat or hamster. TCE containing 0.5-0.6% 1,2-epoxybutane did induce mutagenic responses from strains TA 1535 and TA 100 in the presence and absence of S9 mix. Vapor-phase tests with 1,2-epoxybutane showed that an atmospheric concentration of 0.009% could induce 12-fold and 3-fold increases, respectively, in strains TA 1535 and TA 100. These increases would account for the mutagenic activity of the stabilized TCE sample. The absence of a significant response caused by unstabilized TCE in the presence of S9 mix is probably due to a lack of assay sensitivity, since chloral, a metabolite of TCE, is a mutagen in TA 100.

• OSTI ID: 7190592
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 13:3; ISSN EMMUE; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BODY
CHLORINATED ALIPHATIC HYDROCARBONS
COLONY FORMATION
CYTOLOGICAL TECHNIQUES
DIGESTIVE SYSTEM
GENETIC EFFECTS
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
LIVER
MAMMALS
METABOLIC ACTIVATION
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
RATS
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
VERTEBRATES