Mutagenicity of benzo(a)pyrenyl-1-sulfate in the Ames test

Journal Article · Wed Jan 01 04:00:00 EST 1992 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850190310· OSTI ID:6579699

Irwin, S E; Kwei, G Y; Kauffman, F C ^[1]; Blackburn, G R ^[2]; Thurman, R ^[3]

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1. Rutgers Univ., New Brunswick, NJ (United States)
2. Mobil Environmental and Health Sciences Lab., Princeton, NJ (United States)
3. Univ. of North Carolina, Chapel Hill, NC (United States)

Comparison of the mutagenicity of nine isomeric benzo(a)pyrenyl [B(a)P] phenols conjugated with either sulfate or glucuronide was carried out using strain Salmonella typhimurium TA98. Of the nine conjugates tested, only B(a)P-1-sulfate was mutagenic. Accordingly, the mutagenicity of B(a)P-1-sulfate was compared with that of B(a)P and 1-hydroxybenzo(a)pyrene [B(a)P-1-OH] in the presence and absence of rat lung S9 and Aroclor-induced liver S9 with and without an NADPH-generating system. B(a)P-1-sulfate was slightly mutagenic, whereas B(a)P and the 1-hydroxy derivative were nonmutagenic when S9 fractions and NADPH were omitted. Addition of induced liver S9 with NADPH caused mutagenicity with B(a) -1-OH>B(a)P>B(a)P-1-sulfate. B(a)P-1-sulfate was the only mutagenic species when lung S9 was added. This mutagenicity did not require NADPH. Sodium sulfite, an inhibitor of arylsulfatase, decreased the mutagenicity of B(a)P-1-sulfate. These data suggest that a unique mutagenic species is generated from B(a)P-1-sulfate via arylsulfatase in rat lung. 20 refs., 5 figs., 3 tabs.

• OSTI ID: 6579699
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 19:3; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BENZOPYRENE
CHEMICAL REACTIONS
CONDENSED AROMATICS
DERIVATIZATION
HYDROCARBONS
MUTAGENESIS
ORGANIC COMPOUNDS