Characterization of inositol phosphates in carrot (Daucus carota L. ) cells

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.89.1.126· OSTI ID:7190395

Rincon, M; Chen, Q; Boss, W F ^[1]

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1. North Carolina State Univ., Raleigh (USA)

We have shown previously that inositol-1,4,5-trisphosphate (IP{sub 3}) stimulates an efflux of {sup 45}Ca{sup 2+} from fusogenic carrot protoplasts. In light of these results, we suggested that IP{sub 3} might serve as a second messenger for the mobilization of intracellular Ca{sup 2+} in higher plant cells. To determine whether or not IP{sub 3} and other inositol phosphates were present in the carrot cells, the cells were labeled with myo-(2-{sup 3}H)inositol for 18 hours and extracted with ice-cold 10% trichloroacetic acid. The inositol metabolites were separated by anion exchange chromatography and by paper electrophoresis. We found that ({sup 3}H)inositol metabolites coeluted with inositol bisphosphate (IP{sub 2}) and IP{sub 3} when separated by anion exchange chromatography. However, we could not detect IP{sub 2} or IP{sub 3} when the inositol metabolites were analyzed by paper electrophoresis even though the polyphosphoinositides, which are the source of IP{sub 2} and IP{sub 3}, were present in these cells. Thus, ({sup 3}H)inositol metabolites other than IP{sub 2} and IP{sub 3} had coeluted on the anion exchange columns. The data indicate that either IP{sub 3} is rapidly metabolized or that it is not present at a detectable level in the carrot cells.

• DOE Contract Number: FG05-87ER13693
• OSTI ID: 7190395
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 89:1; ISSN PLPHA; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
BIOCHEMICAL REACTION KINETICS
CALCIUM COMPOUNDS
CARBOHYDRATES
CARROTS
FOOD
HYDROGEN COMPOUNDS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METABOLISM
METABOLITES
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PHYSIOLOGY
PLANT CELLS
PLANTS
REACTION KINETICS
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEGETABLES