Myo-Inositol trisphosphate mobilizes calcium from fusogenic carrot (Daucus carota L. ) protoplasts
To determine whether or not inositol trisphosphate (IP/sub 3/) mobilizes calcium in higher plant cells; they investigated the effect of IP/sub 3/ on Ca/sup 2 +/ fluxes in fusogenic carrot (Daucus carota L.) protoplasts. The protoplasts were incubated in /sup 45/Ca/sup 2 +/-containing medium and the /sup 45/Ca/sup 2 +/ associated with the protoplasts was monitored with time. Addition of IP/sub 3/ (20 micromolar) caused a 17% net loss of the accumulated /sup 45/Ca/sup 2 +/ within 4 minutes. There was a reuptake of /sup 45/Ca/sup 2 +/ and the protoplasts recovered to their initial value by 10 minutes. Phytic acid (IP/sub 6/), also stimulated /sup 45/Ca/sup 2 +/ efflux from the protoplasts. Both the IP/sub 3/- and the IP/sub 6/-induced /sup 45/Ca/sup 2 +/ efflux were inhibited by the calmodulin antagonist, trifluoperazine.
- Research Organization:
- North Carolina State Univ., Raleigh
- OSTI ID:
- 6793033
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 83:2; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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IP/sub 3/ stimulates CA/sup + +/ efflux from fusogenic carrot protoplasts
Inositol trisphosphate metabolism in carrot (Daucus carota L. ) cells
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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARROTS
CATIONS
CELL CONSTITUENTS
CHARGED PARTICLES
CHLOROPLASTS
DAYS LIVING RADIOISOTOPES
ESTERS
EVEN-ODD NUCLEI
FOOD
FUNCTIONS
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
MEMBRANE TRANSPORT
NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PLANTS
RADIOISOTOPES
TRACER TECHNIQUES
VEGETABLES