Changes in inositol phosphates in wild carrot cells upon initiation of cell wall digestion
Conference
·
· Plant Physiol., Suppl.; (United States)
OSTI ID:5520561
Previous studies have shown that inositol trisphosphate (IP/sub 3/) stimulated /sup 45/Ca/sup +2/ efflux from fusogenic carrot protoplasts and it was suggested that IP/sub 3/ may serve as a second messenger for the mobilization of intracellular Ca/sup +2/ in higher plant cells. To determine whether or not inositol phosphate metabolism changes in response to external stimuli, the cells were labeled with myo-(2-/sup 3/H) inositol for 18 h and exposed to cell wall digestion enzymes, Driselase. The inositol phosphates were extracted with ice cold 10% TCA and separated by anion exchange chromatography. The radioactivity of the fraction that contained IP/sub 3/ increased 2-3.8 fold and that which contained inositol bisphosphate increased 1.9-2.6 fold within 1.5 min of exposure to Driselase. After 6 min, the radioactivity of both fractions increased 6-7.7 fold and an increase in inositol monophosphate was observed. These data indicate that inositol phosphate metabolism is stimulated by Driselase and suggest polyphosphoinositide hydrolysis occurs upon initiation of cell wall digestion.
- Research Organization:
- North Carolina State Univ., Raleigh
- OSTI ID:
- 5520561
- Report Number(s):
- CONF-8707108-
- Conference Information:
- Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization of inositol phosphates in carrot (Daucus carota L. ) cells
Myo-Inositol trisphosphate mobilizes calcium from fusogenic carrot (Daucus carota L. ) protoplasts
IP/sub 3/ stimulates CA/sup + +/ efflux from fusogenic carrot protoplasts
Journal Article
·
Sat Dec 31 23:00:00 EST 1988
· Plant Physiology; (USA)
·
OSTI ID:7190395
Myo-Inositol trisphosphate mobilizes calcium from fusogenic carrot (Daucus carota L. ) protoplasts
Journal Article
·
Sat Jan 31 23:00:00 EST 1987
· Plant Physiol.; (United States)
·
OSTI ID:6793033
IP/sub 3/ stimulates CA/sup + +/ efflux from fusogenic carrot protoplasts
Conference
·
Mon Mar 31 23:00:00 EST 1986
· Plant Physiol., Suppl.; (United States)
·
OSTI ID:5021672
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
CALCIUM
CARBOHYDRATES
CARROTS
CATIONS
CELL CONSTITUENTS
CELL WALL
CHARGED PARTICLES
CHROMATOGRAPHY
DIGESTION
ELEMENTS
FOOD
INOSITOL
INOSITOLS
ION EXCHANGE CHROMATOGRAPHY
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PLANT CELLS
PLANTS
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEGETABLES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
CALCIUM
CARBOHYDRATES
CARROTS
CATIONS
CELL CONSTITUENTS
CELL WALL
CHARGED PARTICLES
CHROMATOGRAPHY
DIGESTION
ELEMENTS
FOOD
INOSITOL
INOSITOLS
ION EXCHANGE CHROMATOGRAPHY
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PLANT CELLS
PLANTS
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEGETABLES