Uptake and utilization of CDP-choline in primary brain cell cultures from fetal brain

Journal Article · Mon Feb 28 23:00:00 EST 1983 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00965723· OSTI ID:7190386

Vecchini, A; Binaglia, L; Floridi, A; Palmerini, C A; Procellati, G

The utilization of double-labeled CDP-choline by cultured brain cells has been studied. CDP-choline is demonstrated to be rapidly hydrolysed into CMP and choline phosphate. The fragments, or their hydrolysis products, penetrate into the cells and are utilized for lipid synthesis. At short times after the isotope administration a rapid labeling of phosphatidylcholine was detected, when cells were incubated with CDP-choline. The same was not seen when cells were incubated with labeled choline. From these observations it can be inferred that either CDP- choline can penetrate the cell membrane or that some mechanism involving CDP-choline and leading to phospholipid synthesis can work at the external surface of the plasma membranes.

• Research Organization: Univ. of Perugia, Italy
• OSTI ID: 7190386
• Journal Information: Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 8:3; ISSN NERED
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
CHOLINE
DRUGS
FETUSES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
MEMBRANES
METABOLISM
NERVE CELLS
NUCLEOTIDES
ORGANIC COMPOUNDS
QUATERNARY COMPOUNDS
RATS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES