Metabolism of ethanol to the 1-hydroxyethyl radical by rat heart microsomes

Journal Article · Wed Feb 08 23:00:00 EST 1989 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA)

OSTI ID:7190299

Reinke, L A; Rau, J M; Lai, E K; McCay, P B ^[1]

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1. Oklahoma Health Science Center, Oklahoma City (USA)

Sarcoplasmic reticulum fractions were isolated from rat heart homogenates by differential centrifugation. The membrane fractions were incubated with ethanol (50 mM), an NADPH-generating system, and either 5,5-dimethyl-1-pyrroline N-oxide (DMPO) or N-t-butyl-phenylnitrone (PBN) as spin-trapping agents. Benzene extracts of the reactions were analyzed by electron spin resonance spectroscopy. The rat heart microsomes metabolized ethanol to a carbon-centered radical which formed adducts with both DMPO and PBN. When the experiments were performed with 1-{sup 13}C-ethanol, the spectra were split by the additional spin of the {sup 13}C, which demonstrates that the radical which had been trapped was the 1-hydroxyethyl radical. Heat inactivation of the microsomes resulted in loss of the signal. When the NAPH-generating system was replaced with NADH (1 mM), the intensity of the radical signal decreased by more than 50%. The intensity of the 1-hydroxyethyl radical was increased by more than two-fold by the addition of ADP-Fe{sup +3} or sodium azide, suggesting that iron-catalyzed formation of oxygen radicals may participate in the conversion of ethanol to a free radical under these conditions.

• OSTI ID: 7190299
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (USA) Vol. 3:3; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBON 13
CARBON ISOTOPES
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
COENZYMES
ENDOPLASMIC RETICULUM
ETHANOL
EVEN-ODD NUCLEI
HEART
HOMOGENATES
HYDROXY COMPOUNDS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
METABOLISM
MICROSOMES
NADH2
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
RADICALS
RATS
REACTION KINETICS
RIBOSOMES
RODENTS
SARCOPLASMIC RETICULUM
STABLE ISOTOPES
VERTEBRATES