Free radical metabolism of ethanol by deermouse microsomes

Conference · Thu Mar 14 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5213745

Knecht, K T; Thurman, R G; Mason, R P ^[1]

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1. Univ. of North Carolina, Chapel Hill (United States) NIEHS, Research Triangle Park, NC (United States)

One-electron oxidation of ethanol to the {alpha}-hydroxyethyl radical by rat liver microsomes was previously found to be cytochrome P-450-dependent in the presence of Desferal. With deermouse microsomes, the formation of {alpha}-hydroxyethyl radical from ethanol was demonstrated with the electron paramagnetic resonance (EPR) technique of spin trapping. Free radical formation was enhanced by pretreatment of the animals with ethanol. Boiling of the microsomes, omission of NADPH, or addition of superoxide dismutase abolished free radical formation, but addition of catalase or azide had no affect. Free radicals from the hydroxyl radical scavenger formate were detected when ethanol in the incubation medium was replaced by formate. When equal concentrations of ethanol and formate were added to microsomes, the intensity of each radical adduct signal was decreased by half. Thus, it is concluded that free radical formation in deermouse microsomes is mediated by a P-450-derived, superoxide-dependent oxidizing species.

• OSTI ID: 5213745
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:5; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
BIOLOGICAL EFFECTS
CATABOLISM
CELL CONSTITUENTS
ELECTRON SPIN RESONANCE
ENZYME ACTIVITY
ENZYMES
ETHANOL
HYDROXY COMPOUNDS
MAGNETIC RESONANCE
MAMMALS
METABOLISM
MICE
MICROSOMES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
RADICALS
RESONANCE
RIBOSOMES
RODENTS
SUPEROXIDE DISMUTASE
VERTEBRATES