Inhibition of rat liver microsomal lipid peroxidation by N-acyldehydroalanines: An in vitro comparative study

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Archives of Biochemistry and Biophysics; (USA)

DOI:https://doi.org/10.1016/0003-9861(89)90492-X· OSTI ID:5406926

Buc-Calderon, P; Roberfroid, M ^[1]

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1. Universite Catholique de Louvain, Brussels (Belgium)

Captodative substituted olefins are radical scavengers which react with free radicals to form stabilized radical adducts. One of those compounds, N-(paramethoxyphenylacetyl)dehydroalanine (AD-5), may react and scavenge both superoxide anion (O-2) and alk-oxyl radicals (RO.), and in this way prevent the appearance of their mediated biological effects. Nitrofurantoin and tert-butyl hydroperoxide were used as model compounds to stimulate free radical production and their mediated lipid peroxidation in rat liver microsomes. In addition, lipid peroxidation was also initiated by exposure of rat liver microsomal suspensions to ionizing radiation (gamma rays). The microsomal lipid peroxidation induced by these chemicals and physical agents was inhibited by the addition of AD-5. These effects were dose-dependent in a millimolar range of concentration. In addition, AD-5 has no effect on microsomal electron transport, showing that NADPH-cytochrome P450 reductase activity was not modified. These data, together with the comparisons of the effects of AD-5 and some antioxidant molecules such as superoxide dismutase, uric acid, and mannitol, support the conclusion that inhibition of lipid peroxidation by AD-5 is the result of its free radical scavenger activity. In addition, the inhibitory effect of AD-5 on microsomal lipid peroxidation was dependent of the nature of the free radical species involved in the initiation of the process, suggesting that O-2 is scavenged more efficiently than RO.

• OSTI ID: 5406926
• Journal Information: Archives of Biochemistry and Biophysics; (USA), Vol. 273:2; ISSN 0003-9861
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKENES
BIOLOGICAL EFFECTS
LIPIDS
METABOLISM
RADICALS
BIOSYNTHESIS
ALANINES
BIOLOGICAL RADIATION EFFECTS
COMPARATIVE EVALUATIONS
DOSE-RESPONSE RELATIONSHIPS
ELECTRON TRANSFER
GAMMA RADIATION
IN VITRO
INHIBITION
LIVER
MICROSOMES
PEROXIDES
RATS
SUPEROXIDE DISMUTASE
URIC ACID
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
IONIZING RADIATIONS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PURINES
RADIATION EFFECTS
RADIATIONS
RIBOSOMES
RODENTS
SYNTHESIS
VERTEBRATES
XANTHINES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 - Chemicals Metabolism & Toxicology