Potentiation of ethanol-induced lipid peroxidation of biological membranes by vitamin C
The hydroxyl free radical (/sup ./OH) was generated by the system of ADP-Fe/sup + +/ and H/sub 2/O/sub 2/, trapped by 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and analyzed by electron spin resonance (ESR) spectroscopy. The addition of vitamin C to the system decreased considerably the amount of hydroxyl adduct of DMPO formed. In the presence of ethanol, the ESR spectrum observed was a composite signal of the hydroxyl and hydroxyethyl (HE) adducts of DMPO. However, in the presence of vitamin C and ethanol, pure HE adduct of DMPO was detected. We also detected the increase in lipid peroxidation in the presence of ethanol and vitamin C. These data lead us to hypothesize that in the biological system, formation of these long-lived HE free radicals may result in membrane damage due to an increase in lipid peroxidation.
- Research Organization:
- Univ. of Missouri, Columbia (USA)
- OSTI ID:
- 6148158
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 43:14; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ASCORBIC ACID
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
ELECTRON SPIN RESONANCE
ETHANOL
HYDROXY COMPOUNDS
LIPIDS
MAGNETIC RESONANCE
MEMBRANES
METABOLISM
ORGANIC COMPOUNDS
RADICALS
RESONANCE
VITAMINS