Involvement of active oxygen in lipid peroxide radical reaction of epidermal homogenate following ultraviolet light exposure

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Journal of Investigative Dermatology; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12478534· OSTI ID:5271166

Nishi, J; Ogura, R; Sugiyama, M; Hidaka, T; Kohno, M ^[1]

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1. Kurume Univ. School of Medicine (Japan)

To elucidate the radical mechanism of lipid peroxidation induced by ultraviolet light (UV) irradiation, an electron spin resonance (ESR) study was made on epidermal homogenate prepared from albino rat skin. The exposure of the homogenate to UV light resulted in an increase in lipid peroxide content, which was proportional to the time of UV exposure. Using ESR spin trapping (dimethyl-1-pyrroline-N-oxide, DMPO), the DMPO spin adduct spectrum of lipid radicals (L.) was measured following UV exposure (DMPO-L.:aN = 15.5 G, aH = 22.7 G), as was the spectrum of DMPO-hydroxyl radical (DMPO-OH, aN = aH = 15.5 G). In the presence of superoxide dismutase, the DMPO spin adduct spectrum of lipid radicals was found to be reduced remarkably. Therefore, it was shown that the generation of the lipid radicals partially involves superoxide anion radicals, in addition to hydroxyl radicals. In the ESR free-radical experiment, an ESR signal appeared at g = 2.0064 when the ESR tube filled with homogenate was exposed to UV light at -150 degrees C. The temperature-dependent change in the ESR free radical signal of homogenate exposed to UV light was observed at temperatures varying from -150 degrees C to room temperature. By using degassed samples, it was confirmed that oxygen is involved in the formation of the lipid peroxide radicals (LOO.) from the lipid radicals (L.).

• OSTI ID: 5271166
• Journal Information: Journal of Investigative Dermatology; (United States), Journal Name: Journal of Investigative Dermatology; (United States) Vol. 97:1; ISSN 0022-202X; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
ELECTRON SPIN RESONANCE
ELEMENTS
ENZYMES
EPIDERMIS
EPITHELIUM
HYDROXYL RADICALS
IN VITRO
LIPIDS
MAGNETIC RESONANCE
MAMMALS
METABOLISM
NONMETALS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGEN
RADIATION EFFECTS
RADICALS
RATS
RESONANCE
RODENTS
SKIN
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
SYNTHESIS
TEMPERATURE DEPENDENCE
TISSUES
VERTEBRATES