Isolation, characterization, and UV-stimulated expression of two families of genes encoding polypeptides of related structure in human epidermal keratinocytes

Journal Article · Sun May 01 04:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.5.2195· OSTI ID:7189957

Kartasova, T; Van de Putte, P

By screening of a cDNA library made on mRNA isolated from UV-irradiated human epidermal keratinocytes for sequences whose relative concentration increases in the cytoplasm after irradiation, the authors isolated 40 cDNA clones. Here they describe two distinct groups of cDNA clones which do not cross-hybridize to each other but nevertheless encode proteins of very similar primary structure. These polypeptides are small (8 to 10 kilodaltons) and exceptionally rich in proline, cysteine, and glutamine and have similar repeating elements not found elsewhere. The new proteins were designated sprI and sprII (small, proline rich). The presence of prolines and cysteines suggests that they may be either structural proteins with a strong secondary structure or metal-binding proteins such as metallothioneins. Southern blot and sequence analyses of the cDNAs indicate that at least the sprII group of clones represents a family of related genes. The nucleotide sequence of both groups seems to be conserved upon evolution. The level of mRNAs corresponding to the two groups of cDNAs is increased in the cytoplasm of human epidermal keratinocytes after both UV irradiation and treatment with 4-nitroquinoline 1-oxide or 12-O-tetradecanoylphorbol 13-acetate.

• Research Organization: Building 37, Room 3B25, Lab. of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, MD (US)
• OSTI ID: 7189957
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:5; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400 -- Genetics
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CHEMICAL PROPERTIES
CLONING
DNA
DNA SEQUENCING
DNA-CLONING
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
GENETIC EFFECTS
IRRADIATION
KINETICS
MESSENGER-RNA
METALLOPROTEINS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PHENOTYPE
PROTEINS
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RNA
SEPARATION PROCESSES
SKIN
STRUCTURAL CHEMICAL ANALYSIS
TECHNOLOGY ASSESSMENT
TISSUES
ULTRAVIOLET RADIATION