cDNA cloning of a mouse mammary epithelial cell surface protein reveals the existence of epidermal growth factor-like domains linked to factor VIII-like sequences

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.21.8417· OSTI ID:5821811

Stubbs, J D; Bui, A ^[1]; Lekutis, C; Singer, K L; Srinivasan, U; Parry, G ^[2]; Yuzuki, D ^[3]

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1. Lawrence Berkeley Lab., CA (United States) San Francisco State Univ., CA (United States)
2. Lawrence Berkeley Lab., CA (United States)
3. San Francisco State Univ., CA (United States)

A 2.1-kilobase cDNA coding for a surface protein of mammary epithelial cells has been isolated from a mouse mammary gland {lambda}gt11 cDNA library. Sequence analysis of this cDNA reveals an open reading frame of 1,389 base pairs that defines a protein with a molecular mass of 51.5 dKa. Structural analysis of the predicted sequence identifies two putative functional domains of the protein: (i) an N-terminal cysteine-rich region that is similar to epidermal growth factor-like domains of Drosophila Notch-1 protein and (ii) a large segment of the sequence that exhibited 54.5% identify with C-terminal domains of human coagulation factors VIII and V. These similarities in structure are used to predict the possible functions of the protein and its means of interaction with the cell surface. mRNA expression was detectable in mammary tissue from nonpregnant animals but was maximal in the lactating gland. In cultured cells, mRNA levels also correlated with the degree of cellular differentiation.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5821811
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:21; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL TISSUES
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIPTERA
DNA
DNA SEQUENCING
DROSOPHILA
EPIDERMIS
EPITHELIUM
FLIES
FRUIT FLIES
GLANDS
INSECTS
INVERTEBRATES
ISOTOPES
LACTATION
LIGHT NUCLEI
MAMMALS
MAMMARY GLANDS
MEMBRANE PROTEINS
MESSENGER-RNA
MICE
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
RODENTS
SKIN
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
VERTEBRATES