Novel protein in human epidermal keratinocytes: regulation of expression during differentiation

Journal Article · Sun May 01 04:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.5.2204· OSTI ID:6896187

Kartasova, T; van Muijen, G N; van Pelt-Heerschap, H; van de Putte, P

Recently, two groups of cDNA clones have been isolated from human epidermal keratinocytes; the clones correspond to genes whose expression is stimulated by exposure of the cells to UV light or treatment with 4-nitroquinoline 1-oxide or 12-O-tetradecanoylphorbol 13-acetate. The proteins predicted by the nucleotide sequence of both groups of cDNAs are small (8 to 10 kilodaltons), are exceptionally rich in proline, glutamine, and cysteine, and contain repeating elements with a common sequence, PK PEPC. These proteins were designated sprI and sprII (small, proline rich). Here we describe the characterization of the sprIa protein, which is encoded by one of the group 1 cDNAs. The expression of this protein during keratinocyte differentiation in vitro and the distribution of the sprIa protein in some human tissues was studied by using a specific rabbit antiserum directed against a synthetic polypeptide corresponding to the 30 amino acids of the C-terminal region of the sprIa gene product. The results indicate that the expression of the sprIa protein is stimulated during keratinocyte differentiation both in vitro and in vivo.

• Research Organization: State Univ. of Leiden (Netherlands)
• OSTI ID: 6896187
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:5; ISSN MCEBD
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARCINOGENS
CARDIOVASCULAR SYSTEM
CELL CULTURES
CELL DIFFERENTIATION
CLONE CELLS
DIGESTIVE SYSTEM
DNA
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
ESOPHAGUS
ESTERS
GENE REGULATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HEART
HETEROCYCLIC COMPOUNDS
MUSCLES
MYOCARDIUM
NITRO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
PHORBOL ESTERS
PROTEINS
PYRIDINES
QUINOLINES
RADIATION EFFECTS
RADIATIONS
SKIN
TISSUES
ULTRAVIOLET RADIATION