Circulating factors that modify lung cell DNA synthesis following exposure to inhaled oxidants. II. Effect of serum and lavage on lung pneumocytes following exposure of adult rats to 1 ppm ozone

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Journal of Toxicology and Environmental Health; (USA)

DOI:https://doi.org/10.1080/15287399009531377· OSTI ID:7189546

Tanswell, A K; Fraher, L J; Grose, E C ^[1]

---

1. Univ. of Western Ontario, London (Canada)

Adult rats were exposed to 1 ppm (1.96 mg/m3) ozone or air for 2 wk. Animals were sacrificed at 3, 5, 7, or 14 d after the onset of exposure, and samples of plasma and lung lavage were obtained. Heat-inactivated plasma and lavage from animals exposed to ozone for 5 or 7 d significantly increased DNA synthesis by lung pneumocytes compared with plasma or lavage from air-exposed animals. Fractionation of plasma and lavage samples indicated that the factor responsible had an isoelectric point of 6.45-6.75, and a molecular weight of 38 +/- 3 kDa. This factor has a dose-dependent effect on lung pneumocyte DNA synthesis in culture. It has no effect on cultured fibroblast DNA synthesis, and is distinct from a previously described factor in the plasma of these ozone-exposed animals that enhances fibroblast DNA synthesis. The factor is detectable within 5 d of exposure, and may hold some promise as a marker of early oxidant lung injury.

• OSTI ID: 7189546
• Journal Information: Journal of Toxicology and Environmental Health; (USA), Journal Name: Journal of Toxicology and Environmental Health; (USA) Vol. 29:1; ISSN JTEHD; ISSN 0098-4108
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
CONNECTIVE TISSUE CELLS
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
INHALATION
INTAKE
LAVAGE
LUNGS
MAMMALS
MOLECULAR WEIGHT
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OZONE
RATS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
TOXICITY
VERTEBRATES